![]() The following table lists the character rosters for Dynasty Warriors: Strikeforce as well as Shin Sangoku Musou Multi Raid 2, the latter of which does not see release beyond Japan. Before participating in a mission players can rest or undergo preparation in a small hub complete with an academy, workshop, blacksmith, storehouse, and shrine. The multiplayer mode allows you to pair up with up to three other players to participate together in raids and even against each other. The handheld version of Dynasty Warriors would also introduce ad hoc online multiplayer to the series. Strikeforce would introduce a more RPG style of leveling where characters would get a small increase in stats as they leveled up to a cap of 50 weapons also received a leveling system. Orbs give special bonuses to a weapon, and can increase attack power, range, and more while Chi items give unique abilities such as additional jumps, stronger attacks, Fury form usage reduction, and actual floating. Characters saw more capability for customization, as in addition to the ability to equip weapons and accessories seen in the previous franchise entries, characters could now equip a sub-weapon (with no restriction on weapon type by character) which can be switched to with the circle button, along with orbs and chi to alter stats. The Awakening drastically affects the appearance of the character and increases their stats.
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![]() The image shows the separation pattern for nine different proteins for each acrylamide concentration. High molecular weight proteins will be optimally resolved in gels containing a lower acrylamide content, while smaller proteins should ideally be run in more acrylamide-dense gels. When selecting a gel, it is important to use an acrylamide concentration that will allow optimal separation of the proteins in your sample. At the end of the separation, the molecules are detected as bands at different positions in the matrix. Differently sized, shaped, and charged molecules in the sample move through the matrix at different velocities. This then aids the selection process when considering the conditions that will enable you to most effectively get the most information you require from your specific analysis.Įlectrophoresis is normally carried out by loading a sample containing the molecules of interest into a well in a porous matrix (polyacrylamide) to which a voltage is then applied. It is used mainly for the analysis and purification of large molecules such as proteins or nucleic acids. Electrophoresis is a separation technique based on the mobility of charged molecules in an electric field. Our SDS-PAGE Clean-Up Kit is designed for the preparation of samples that are difficult to analyze due to the presence of salts or a low protein concentration.Įlectrophoresis is the second step,step and is a commonly used method for separating proteins based on size, shape, and/or charge. However, sample cleanup improves performance by removing potentially interfering compounds such as nucleic acids, polysaccharides, and salts. It is not usually necessary to treat samples prior to 1-D gel electrophoresis. Extract proteins quickly, on ice, if possible, in the presence of a suitable buffer to maintain pH, ionic strength, and stability in order to prevent protein degradation. Use extraction procedures that are as mild as possible. Numerous methods are available for disrupting cells and preparing their contents for analysis by Western blotting. The target protein is likely to be one among many thousands present in the sample, in addition to nucleic acids, polysaccharides, and lipids, all of which might interfere with the analysis. The process begins with the sample of interest usually undergoing some degree of preliminary treatment before continuing to separation by electrophoresis. In this guide, we’ll explore these seven steps to Western blot.ĭid you know: First described in 1979, the method of Western blotting has since become one of the most commonly used methods in life science research. The method is based on building an antibody:protein complex via specific binding of antibodies to proteins immobilized on a membrane and detecting the bound antibody with one of several detection methods.Īlthough the details of Western blotting protocols may vary from among applications, with adaptations to suit specific protein characteristics and the level of information required, they all follow some common basic steps. Western blotting, also known as immunoblotting, is a well-established and widely used technique for the detection and analysis of proteins. ![]() ![]() ![]() Unlike many of the other options on this list, you can still buy it new, which makes the Unihertz Titan a strong contender. That’s a newer OS than the Key2, but hardly anything to get excited about. The camera is just okay, and the phone is apparently stuck with Android 9.0 Pie now. A 6,000mAh battery makes the most of the phone’s bulk, and we saw some great performance on standby. ![]() Gaming isn’t likely to be something you’re going to want to do on this phone, though, which is generally true for most QWERTY phones anyway. We had a few hangups, but generally, the performance was solid. The processor is the MediaTek Helio P60, and like most processors you’ve probably not heard of, it’s a decent, if not a noteworthy, performer. ![]() It’s a square 1400 x 1400 resolution display, and while it’s nothing special, it certainly does the job. Still, we imagine most people will need a period of adaptation when using a QWERTY phone these days, so perhaps this won’t be as big an issue for some. You’ll need to tap a software bar at the bottom of the screen for punctuation, and some keys are in odd places, which makes the typing experience feel slightly odd. The keyboard also isn’t as good as the Key2’s. This isn’t a phone for your pocket, and it shows. 303 grams is an awful lot in a phone, and you can really tell when you’ve been using this for a while. It’s almost a full inch wider than the BlackBerry Key2, twice as thick, and almost twice as heavy to boot. The rugged nature is backed up by a truly enormous build. Metal plates cover the sides of the phone and the camera lens, and a thick rubber coating covers everything else. The sturdy construction is one of the keystone features of this phone. Yes, you read that right - a rugged QWERTY phone. Who it’s for: Someone who commonly finds themselves typing emails in challenging environments. Why you should buy this: You need a QWERTY phone with a bit more protection than your usual slim, sleek business phone. Read our full BlackBerry Key2 review Best rugged QWERTY phone: Unihertz Titan The screen is only 4.5 inches to accommodate the keyboard, and it’s an odd aspect ratio to boot.īut even with these problems, the BlackBerry Key2 is still the best overall QWERTY phone we can recommend. The camera suffers from shutter lag and mixed low-light performance, but the Portrait Mode is good. ![]() Performance wasn’t too far from the flagship phones of the time, but the gulf has widened since then, and you’ll find that this one definitely lags behind more modern phones. You can still program button functions, but there’s also a Speed key now, which acts as a shortcut into apps.ĭo you have to compromise to get a keyboard like this? Unfortunately, yes. The backlit keyboard is also capacitive, which means it’s touch-sensitive, so you can flick up to select suggested words on the predictive bar, and you can use the whole keyboard as a trackpad for scrolling. BlackBerry refined the keyboard experience offered by the original KeyOne by adding slightly larger keys and dropping the frets between them to provide more room. Sporting the best keyboard experience you’ll find on a smartphone today, this is still an attractive, moderately powerful phone with plenty of stamina and a host of security and privacy features. As such, this isn’t a phone to buy if getting the latest OS matters to you.īut despite those hefty downsides, the BlackBerry Key2 remains an excellent piece for QWERTY typing. Who it’s for: Anyone who needs the best QWERTY phone around.īlackberry hasn’t shown much interest in upgrading the line for a Ke圓, and it hasn’t even bothered to update the phone from Android 8.1 Oreo - an operating system update released in December 2017. Why you should buy this: It’s still the best choice for a QWERTY keyboard-equipped smartphone, even if it is no longer updating.
![]() The Feudal System greatly affected daily life for everyone, but, in time, it collapsed. ![]() William introduced feudalism to England in 1066. All the different small problems added to the confusion and replacement of feudalism by The Renaissance. The word feudalism comes from the word fief, which was the land held on condition of feudal service, similar to an estate (English). Feudalism was a system of government that developed in the Medieval Ages. And with this the higher classes helped the lower classes with protection and land. Serfs were similar to slaves in the Roman Empire (Cels 8). A: Feudalism, short for the feudal system, has long reaching roots that can be best described as vague in its founding. Feudalism started in the definite form in the lands of Frankish in the century of the 9th and the 10th. ![]() The farmers would then grow and harvest crops on these very small strips of land (King 34). The feudal system had been used in France by the Normans from the time they first settled there in about 900 AD. Also, it meant he could reward his supporters at the same time as ensuring they stayed loyal. One of the determining factors in the downfall of feudalism was the Hundred Years War. After defeating the king, William I tried to centralize the power so that he could not be defeated by any other king. In our day and time Feudalism would be difficult and baffling, Feudalism a social system of duties and rights on land tenure and personal relationships in fief lands by vassals that came from lords to which they owe very specific services, and to which they are bounded by personal loyalty. The freemen and the serfs provided the manor with specialists who were essential to the economy. William used the methods of control that he was most familiar with: castles and the feudal system. The last large step in the diffusion of feudalism was in 1066 when the normans took, organization called feudalism was a way of life that had great effect on people of the time and on the modern world. Feudalism arose following the fall of the Roman Empire. He owned all the land in the country and decided who he would lease land to. The lord could sell the serf’s land and marry them to whoever the lord pleased or even separate their families (Bishop 230). The Feudal system was introduced to England and spread throughout Britain by William the Conqueror after 1066 and the Battle, Quarterly Assessment Feudalism was the overarching…, Feudalism was a unique form of government that unified the people of medieval Europe. The feudal system was William's way of splitting the land between the people of England. The vassals then need to serve his lord faithfully (Nardo 25). “Feudalism” is not a medieval term and not even a translation of a medieval concept (Abels 2010 Brown 2010 Bouchard 1998). Feudalism and Capitalism The Feudal system helped William the Conqueror consolidate his power over England. Freemen were the legally privileged (Bishop 229). William was first a conqueror, and he defeated the English army led by Harold. from this period that survives and can be deciphered. The Feudal System was created by William the Conqueror. ![]() I would…, Feudalism made it 's way when the Carolingian dynasty started to gain more land. In order to attempt to answer a question such as this we must briefly describe the Feudal system then look at the advantages of having feudalism as a system and also the disadvantages and then secondly look at the alternatives if there was no such system in place. William the first brought this form of government to England after he defeated Harold at the Battle of Hastings. William invented the Fuedal system, The King was at the top of the Feudal system then the Nobles, then the Knights and finally the Peasants at the bottom. Finally, I will outline some of the advantages and disadvantages of the feudal system. The reason it started was because people needed a source of protection. ![]() ![]() ![]() The standard error output I'm getting is (spacing added): I've run into a wall and can't get the app (anaconda navigator) to launch. ![]() Raw_data = collections.OrderedDict(kv for kv in, I'm a Linux newbie trying to install/run Anaconda Navigator for the purpose of learning Python. ![]() : unacceptable character #x0000: special characters are not allowed in "C:\Users\Admin\.continuum\anaconda-client\config.yaml ", position 0 Conda list Self._client_api = ClientAPI(config=nfig)įile "E:\Anaconda\lib\site-packages\anaconda_navigator\api\client_api.py ", line 683, in ClientAPIįile "E:\Anaconda\lib\site-packages\anaconda_navigator\api\client_api.py ", line 145, in _init_įile "E:\Anaconda\lib\site-packages\anaconda_navigator\api\client_api.py ", line 356, in reload_client acker = GATracker() if analytics else Noneįile "E:\Anaconda\lib\site-packages\anaconda_navigator\utils\analytics.py ", line 253, in GATrackerįile "E:\Anaconda\lib\site-packages\anaconda_navigator\utils\analytics.py ", line 89, in _init_įile "E:\Anaconda\lib\site-packages\anaconda_navigator\api\anaconda_api.py ", line 1176, in AnacondaAPIįile "E:\Anaconda\lib\site-packages\anaconda_navigator\api\anaconda_api.py ", line 71, in _init_ Unacceptable character #x0000: special characters are not allowed in "C:\Users\Admin\.continuum\anaconda-client\config.yaml ", position 0ĭuring handling of the above exception, another exception occurred:įile "E:\Anaconda\lib\site-packages\anaconda_navigator\exceptions.py ", line 87, in handle_exceptionįile "E:\Anaconda\lib\site-packages\anaconda_navigator\exceptions.py ", line 19, in display_qt_error_boxįile "E:\Anaconda\lib\site-packages\anaconda_navigator\widgets\dialogs\_init_.py ", line 530, in _init_įile "E:\Anaconda\lib\site-packages\anaconda_navigator\widgets\dialogs\_init_.py ", line 338, in _init_ : unacceptable character #x0000: special characters are not allowed in "C:\Users\Admin\.continuum\anaconda-client\config.yaml ", position 0 Raise ReaderError(self.name, position, ord(character), Raw_data = collections.OrderedDict(kv for kv in _iterable(load_paths))įile "E:\Anaconda\lib\site-packages\binstar_client\utils\config.py ", line 242, in įile "E:\Anaconda\lib\site-packages\binstar_client\utils\config.py ", line 222, in _dir_yaml_loaderįile "E:\Anaconda\lib\site-packages\binstar_client\utils\config.py ", line 206, in load_configįile "E:\Anaconda\lib\site-packages\binstar_client\utils\yaml.py ", line 12, in yaml_loadįile "E:\Anaconda\lib\site-packages\yaml\_init_.py ", line 125, in safe_loadįile "E:\Anaconda\lib\site-packages\yaml\_init_.py ", line 79, in loadįile "E:\Anaconda\lib\site-packages\yaml\loader.py ", line 34, in _init_įile "E:\Anaconda\lib\site-packages\yaml\reader.py ", line 85, in _init_įile "E:\Anaconda\lib\site-packages\yaml\reader.py ", line 135, in determine_encodingįile "E:\Anaconda\lib\site-packages\yaml\reader.py ", line 169, in updateįile "E:\Anaconda\lib\site-packages\yaml\reader.py ", line 143, in check_printable ![]() File "E:\Anaconda\lib\site-packages\anaconda_navigator\exceptions.py ", line 74, in exception_handlerįile "E:\Anaconda\lib\site-packages\anaconda_navigator\app\start.py ", line 137, in start_appįile "E:\Anaconda\lib\site-packages\anaconda_navigator\app\start.py ", line 59, in run_appįile "E:\Anaconda\lib\site-packages\anaconda_navigator\widgets\main_window\_init_.py ", line 217, in _init_įile "E:\Anaconda\lib\site-packages\anaconda_navigator\utils\anaconda_solvers\core.py ", line 76, in solveĬonfiguration: typing.Any = binstar_config()įile "E:\Anaconda\lib\site-packages\binstar_client\utils\config.py ", line 250, in get_configįile_configs = load_file_configs(SEARCH_PATH)įile "E:\Anaconda\lib\site-packages\binstar_client\utils\config.py ", line 242, in load_file_configs ![]() ![]() ![]() So, these steps would turn off mouse acceleration in Windows 10 and 7. Should I Turn Off Mouse Acceleration If you’re wondering whether Windows 10 has enabled mouse acceleration, you’ll need to change the settings to make sure that. ![]() Click on the Enable pointer precision checkbox. This poorly understood feature is enabled by default in Windows and is a form of mouse acceleration. To enable mouse acceleration, go to the Pointer Options tab of the Control Panel and click on the Pointer Precision section. The “Enhance Pointer Precision” feature in fact makes your mouse movement less precise in many situations. Is Windows mouse acceleration on by default? Now, uncheck or untick the box “Enhance Pointer Precision”.In Mouse Properties, switch to the Pointer Options tab. Right-click on your mouse, and select Mouse Settings. Under Hardware and Sound, select View Devices and Printers. Turning off mouse acceleration in Windows 10 isn’t hard: Open the Control Panel. Navigate to Pointer Options tab in the Mouse Properties window How to Turn Off Mouse Acceleration in Windows 10.Select “mouse” under the ‘device and printers’ section.Next up, tap the ‘Hardware and Sound’ option.Note: These steps work perfectly fine for Windows 10 and Windows 7.ĭisable Mouse Acceleration through Control Panel Window How to turn off mouse acceleration in Windows 10 Mouse acceleration is turned on by default in Windows 10. Let’s start: How to Completely Disable Mouse Acceleration in Windows 10 & 7įollow these steps to turn off Enhance pointer precision for better aim and ultra-precision: Here’s how you can turn off mouse acceleration on Windows 10 or 7 step-by-step so the next time you have an edge to reach that next rank in Overwatch or CS: GO. The worst part: A little-known feature with which we have struggled to correct for far has messed with us and threw us all off while playing. The best part: It’s super simple to completely disable mouse acceleration in Windows 10 or Windows 7. ![]() ![]() ![]() Great Lakes Dredge & Dock Corporation (NASDAQ:GLDD) has signed a contract with Conrad Shipyard for the construction of a 6,500-cubic-yard-capacity trailing suction hopper dredge. Mackie and its 15,000-cubic-yard-capacity barge Ellis Island. Great Lakes Dredge & Dock’s existing six-dredge hopper fleet includes the ATB tug Douglas B. Inmarsat marks a Fleet Xpress milestone and launches a data academy.Dropping in bio-LNG extends green financing runway says SEA-LNG.VesselsValue: Volatility continues in crude tanker sector.Op-Ed: Shipping’s next fuels must be assessed from well to wake.Op-Ed: The future of maritime connectivity to enable digital transformation.Ambitious green goals or logistics headaches?.Is the criminalization of seafarers on the rise?.Recovery efforts underway in path of hurricane.Corps releases inland waterways spend plan.Q&A: 10 minutes with OneOcean on its path to sustainability.CEO Spotlight: John Waterhouse, founder & principal of Elliott Bay Design Group.A Q&A with ferry attorneys Katherine M.Shipping losses fall, but that’s not the whole story.Salvage industry sees only “modest” recovery in gross revenues.Resolve Pioneer set for 5 more years of service in Western Alaska.Op-Ed: Ports risk being left on the wrong side of the great sustainability divide.Crowley names Atkinson VP of sustainability.Op-Ed: Marine industry embraces sustainability with minimal impacts to capital budgets.ICS calls for global CO2 reduction fund to reward green fuel first users.Maersk: Record results, but “dark clouds” warning.Royal Caribbean reports a “better than expected” quarter.NTSB reports on $1.8 million towboat engineroom fire.USCG: Don’t over estimate DPS systems capabilities.Insurers release white paper on lithium-ion battery transportation risks.OFAC slaps sanctions on FBI-wanted shipowner.Op-Ed: Knowledge and planning are critical CII and EEXI compliance tools.Mayorkas faces Congressional ire over Jones Act waivers.“Tougher than IMO” bill on ship emissions introduced in House.GAO report looks at Navy multiyear procurements.NOIA praises Manchin’s Inflation Reduction Act provisions.clean shipping legislation wins support from Maersk Bearing launches AI product to predict and manage impact of CII.IMO: WSC weighs in on what should follow MEPC 78.AMP sets record straight on need for Puerto Rico Jones Act waiver.Liner operators ask EU to extend vessel sharing exemption.260 jobs created with new $35 million facility in Henderson County.Q&A: How ship-to-shore power decarbonization technology is changing the maritime industry. ![]()
![]() ![]() Sometimes it's terribly hard to trust God. I realize, of course, that trusting in the Lord is not always easy. Google follows its corporate dictum, "Don't be evil." We follow the Lord's directive, "Do good." We use well the gifts and talents God has given us, whether at work or at school, in our church or our community, in our family or among our friends. We feed the hungry and seek justice for the oppressed. ![]() We love our neighbors and even our enemies. We do the good that God has shown us in Scripture. We trust in the Lord because he has revealed himself to be trustworthy. It involves leaning back into his arms, receiving his grace, and putting our hope in him. Trusting in God is far more than mere belief. It means hearing his directives and following them obediently. Then you will live safely in the land and prosper." That's about as simple as it gets: "Trust in the LORD and do good."įirst, we trust in the Lord. But perhaps the simplest guidance for living well comes in verse 3: "Trust in the LORD and do good. "Be still in the presence of the Lord" (v. "Commit everything you do to the Lord" (v. It serves up plenty of good advice for living: "Don't worry about the wicked" (v. There's just "too much information." Wouldn't it be great if life could be made, well, real simple? That's just what I need these days, as my life seems to be getting ever more complicated, with a to-do list that never ends and an email inbox that never remains empty. The magazine's subtitle is just as alluring as the title: "Life Made Easier." Sounds good, doesn't it? Real Simple. If you're a woman, you may even have bought a copy. Trust in the LORD and do good. Then you will live safely in the land and prosper.Īre you familiar with the magazine called Real Simple? Perhaps you've seen it in the grocery store. Learning From the Psalms How to Pray Through Your Work.Beyond Rank and Power: What Philemon Tells Us About Leadership.Evangelism - Sharing the Gospel at Work.10 Key Points About Work in the Bible That Every Christian Should Know. ![]() ![]() Submarines can now be detected by the tiny amounts of radiation and chemicals they emit, by slight disturbances in the Earth’s magnetic fields, and by reflected light from laser or LED pulses. Today’s sensing technologies for detecting submarines are moving beyond merely hearing submarines to pinpointing their position through a variety of non-acoustic techniques. Rose Gottemoeller, former deputy secretary general of NATO, warns that “the stealth of submarines will be difficult to sustain, as sensing of all kinds, in multiple spectra, in and out of the water becomes more ubiquitous.” And the ongoing contest between stealth and detection is becoming increasingly volatile as these new technologies threaten to overturn the balance. Many naval experts are speculating about sensing technologies that could be used in concert with modern AI methodologies to neutralize a submarine’s stealth. The twin problems of detecting submarines of rival countries and protecting one’s own submarines from detection are enormous, and the technical details are closely guarded secrets. And that’s just the purchase price the total life cycle cost for the new Columbia –class ballistic-missile submarine is estimated to exceed $395 billion. Nuclear-powered submarines, such as the Virginia –class fast-attack submarine, each cost roughly US $2.8 billion, according to the U.S. A submarine that goes into service today should still be in service in 2082. This timing is particularly significant because the enormous costs required to design and build a submarine are meant to be spread out over at least 60 years. It may comeĪs early as 2050, according to a recent study by the National Security College of the Australian National University, in Canberra. And now the game of submarine hide-and-seek may be approaching the point at which submarines can no longer elude detection and simply disappear. ![]() ![]() Radwell's engineering team offers services to upgrade or replace RQANS1 products to RPMFN1 lines.In either event, the unit will go through Radwell's Quality Assurance review This denotes that the product was inspected to ensure quality and authenticity, or it indicates that the previous owner opened the seal. For quality assurance purposes some products may not have a factory seal.The products are genuine, in surplus never used original packaging, but they may be older date codes or series than that available direct from the factory or authorized dealers.Radwell sources these products through independent channels including Original Equipment Manufacturers (OEM), Internet exchanges, direct or independent distributors, miscellaneous resellers, auctions, or direct from user tool cribs.Radwell is not an authorized surplus dealer or affiliate for the Manufacturer of RQANS1 products unless explicitly stated otherwise.Because Radwell is not an authorized distributor of RISCN1 products, the Original Manufacturer's warranty may not apply. All RISCN1 items purchased from Radwell International are covered by Radwell's industry-leading Radwell Quality Assurance ("RQA") 2-Year Warranty.Ships same day if in stock and ordered by 3:00 p.m.Radwell's engineering team offers services to upgrade or replace RISCN1 products to RPMFN1 lines.Radwell's engineering team does not make any recommendations regarding this product.This denotes that the product was inspected to ensure quality and authenticity For quality assurance purposes, some products may not have a factory seal.The products may vary as to country of origin, accessories and other items included with the product, the language used on the packaging, parts and instructions, and the contents of any printed material.The products are genuine, new in box, and unused.Radwell sources these products through independent channels including resellers, the original manufacturer (not as an authorized distributor), and online marketplaces, allowing Radwell to obtain competitive buying rates.Radwell is not an authorized distributor or an affiliate of the Manufacturer of RISCN1 products. ![]() |
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